DNA Methyltransferase 3b Accelerates the Process of Atherosclerosis

Figure 2

The percentages of Treg and inflammatory cytokine levels in peripheral blood of ApoE^−/− mice. . (a) Representative FACS results of Tregs (gated by CD4⁺ T cells) from a single mouse in four groups. Numbers represent the percentage of cells in the quadrants (CD4⁺CD25⁺Foxp3⁺/CD4⁺ T cells). (b) Results of the statistical analysis of peripheral blood Treg among four groups. (c) The correlation between the methylation levels of Foxp3-TSDR and Tregs ratio of peripheral blood in ApoE^−/− mice was calculated by using the Spearman rank correlation coefficient ( = −0.789, ). (d) The plasma concentrations of ox-LDL among four groups. (e) The plasma concentrations of TGF-β among four groups. (f) The plasma concentrations of IL-10 among four groups. Abbreviations: Foxp3: forkhead box P3; ox-LDL: oxidized low-density lipoprotein; TSDR: Treg-specific demethylated region; WD: western diet.

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