DNA Methyltransferase 3b Accelerates the Process of Atherosclerosis

Figure 3

Foxp3-TSDR methylation levels of peripheral blood and FOXP3 expression levels of Treg in ApoE^−/− mice. (a) Methylation status of 10 individual CpG motifs of the Foxp3-TSDR is shown by white (demethylation) or black (methylation) circles. Repeat sequencing for 3 times per mouse. (b) Results of the statistical analysis of Foxp3-TSDR methylation (, repeated 3 times). , . (c) The mRNA expression levels of Dnmt3b in spleen Treg after transduction Dnmt3b-shRNA into ApoE^−/− mice (, repeated 3 times). (d) The mRNA expression levels of Foxp3 in spleen Treg after transduction Dnmt3b-shRNA into ApoE^−/− mice. (e) Western blot results of Dnmt3b and Foxp3 in spleen Treg after transduction Dnmt3b-shRNA into ApoE^−/− mice (, repeated 3 times). (f) The protein expression levels of Dnmt3b in spleen Treg after transduction Dnmt3b-shRNA into ApoE^−/− mice. (g) The protein expression levels of Foxp3 in spleen Treg after transduction Dnmt3b-shRNA into ApoE^−/− mice. Abbreviations: Dnmt3b: DNA methyltransferases 3b; Foxp3: forkhead box P3; TSDR: Treg-specific demethylated region; WD: western diet.

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