Therapeutic Potential of Polyscias fruticosa (L.) Harms Leaf Extract for Parkinson’s Disease Treatment by Drosophila melanogaster Model

Figure 7

P. fruticosa leaf extract reduced degeneration of dopaminergic neurons caused by knockdown of dUCH in larvae. (a–f) Representative immunostaining images of brain lobes using antityrosine hydroxylase antibody. The control (TH > CS) and dUCH-knockdown larvae (TH > dUCH-IR) were treated with 2 mg/mL of PLE and 0.5 mM vitamin C. (A₁-F₁) and (A₂-F₂) show dopaminergic neurons in DM and DL2 clusters with scale bars of 50 and 25 μm, respectively. The quantified data of the numbers of dopaminergic neurons in three clusters (DL1, DL2, and DM) and sum of three clusters was also presented in column charts (g-k). , -test: , , and . The control line TH > CS (+; +; TH-GAL4/+) and the dUCH-knockdown line TH > dUCH-IR (+; +; TH-GAL4/UAS-dUCH-IR). Error bars represent the standard deviation of data. VitC: 0.5 mM vitamin C.