Insulin-Related Liver Pathways and the Therapeutic Effects of Aerobic Training, Green Coffee, and Chlorogenic Acid Supplementation in Prediabetic Mice

Figure 5

HFD is associated with changes in hepatic metabolic enzyme mRNA levels. (a) RT-qPCR analysis of Gys2 in the livers from the Ctrl and HFD mice. (b) Immunoblotting analyses of p-GYS2 and total GYS2 protein in the livers from the Ctrl and HFD groups, (c) RT-qPCR analysis of G6pc2, and (d) Peck mRNA in the livers from the Ctrl and HFD mice. (e–g) RT-qPCR analysis of Pk, Pfkl, and Gk mRNA in the livers from the Ctrl and HFD mice. All values are presented as . , and indicate significant statistical differences in the HFD compared to Ctrl mice.

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