Insulin-Related Liver Pathways and the Therapeutic Effects of Aerobic Training, Green Coffee, and Chlorogenic Acid Supplementation in Prediabetic Mice

Figure 8

GC, CGA, EX, EX. GC, and EX.CGA treatment alters mRNA expression of multiple metabolic genes in livers from PD mice. (a) RT-qPCR analysis of Gys2 mRNA levels in the liver from PD and all treated animal groups. (b) Immunoblot of protein levels of p-GYS2 and total GYS2 in the liver from PD and all treated groups. (c) RT-qPCR analysis of G6pc2 and (d) Peck mRNA in the livers from PD and all treated animal groups. (e–g) RT-qPCR analysis for Pk, Pfkl, and Gk mRNA levels in the livers from PD and all treated groups. All values are presented as . ,, and indicate significant statistical differences in the treated compared to PD mice.

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