Daurisoline Inhibits ESCC by Inducing G1 Cell Cycle Arrest and Activating ER Stress to Trigger Noxa-Dependent Intrinsic and CHOP-DR5-Dependent Extrinsic Apoptosis via p-eIF2<i>α</i>-ATF4 Axis

<div>Daurisoline induced p21-/p27-dependent G1 phase cell cycle arrest. (a) EC1 and ECA109 cells were incubated with DMSO or DAS for 24 hours, followed by PI staining and FACS analysis for cell cycle profiling. The percentage of each cell cycle was counted to draw a statistical graph. (b) EC1 and ECA109 cells were incubated with DAS at indicated concentrations, and proteins were extracted and detected by western blot. (c-f) EC1 and ECA109 cells were transfected with control or two siRNA sequences of p21 or p27, then treated with DAS (20 <i>μ</i>M) for 24 hours, and finally subjected to PI staining and FACS analysis. The protein levels of p21 or p27 were determined by western blot.</div>

Oxidative Medicine and Cellular Longevity

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Figure 2: Daurisoline Inhibits ESCC by Inducing G1 Cell Cycle Arrest and Activating ER Stress to Trigger Noxa-Dependent Intrinsic and CHOP-DR5-Dependent Extrinsic Apoptosis via p-eIF2<i>α</i>-ATF4 Axis 

Figure 2 | Daurisoline Inhibits ESCC by Inducing G1 Cell Cycle Arrest and Activating ER Stress to Trigger Noxa-Dependent Intrinsic and CHOP-DR5-Dependent Extrinsic Apoptosis via p-eIF2α-ATF4 Axis 