Daurisoline Inhibits ESCC by Inducing G1 Cell Cycle Arrest and Activating ER Stress to Trigger Noxa-Dependent Intrinsic and CHOP-DR5-Dependent Extrinsic Apoptosis via p-eIF2α-ATF4 Axis

Figure 3

Daurisoline induced cell death mainly through caspase-dependent apoptosis. (a) EC1 and ECA109 cells were treated with Z-VAD (20 μM), Nec-1 (50 μM), NSA (10 μM), and Fer-1 (1 μM) or combined with DAS (40 μM) for 24 hours, followed by the ATPlite assay. (b, c) EC1 and ECA109 cells were treated with indicated concentrations of DAS or DAS (40 μM) and Z-VAD (20 μM) for 24 hours, then stained with an Annexin-V-FITC apoptosis detection kit and analyzed with FCAS, the proportion of apoptotic cells was counted, and the statistical graph was made. (d, e) EC1 and ECA109 cells were treated with indicated concentrations of DAS or DAS (40 μM) and Z-VAD (20 μM) for 24 hours, and proteins were extracted and detected by western blot.

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