Daurisoline Inhibits ESCC by Inducing G1 Cell Cycle Arrest and Activating ER Stress to Trigger Noxa-Dependent Intrinsic and CHOP-DR5-Dependent Extrinsic Apoptosis via p-eIF2<i>α</i>-ATF4 Axis

<div>Daurisoline triggered Noxa-dependent intrinsic apoptosis. (a) EC1 and ECA109 cells were treated with DAS as described above and were subjected to western blot. (b) EC1 and ECA109 cells were incubated with DAS at indicated concentrations, and proteins were extracted and detected by western blot (EL: 23 kDa; L: 15 kDa; S: 12 kDa). (c) The mRNA level of Noxa was quantified by Q-PCR. (d, e) EC1 and ECA109 cells were transfected with control or two siRNA sequences of Noxa, then treated with DAS (30 <i>μ</i>M) for 24 hours. Apoptosis induction was quantified by Annexin V-FITC/PI double-staining analysis. Noxa and cleaved PARP protein levels were detected by western blot.</div>

Oxidative Medicine and Cellular Longevity

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Figure 4

Figure 4: Daurisoline Inhibits ESCC by Inducing G1 Cell Cycle Arrest and Activating ER Stress to Trigger Noxa-Dependent Intrinsic and CHOP-DR5-Dependent Extrinsic Apoptosis via p-eIF2<i>α</i>-ATF4 Axis 

Figure 4 | Daurisoline Inhibits ESCC by Inducing G1 Cell Cycle Arrest and Activating ER Stress to Trigger Noxa-Dependent Intrinsic and CHOP-DR5-Dependent Extrinsic Apoptosis via p-eIF2α-ATF4 Axis 