Superoxide Dismutase Prevents SARS-CoV-2-Induced Plasma Cell Apoptosis and Stabilizes Specific Antibody Induction

Figure 2

Assessment of CoV-specific plasma cell apoptosis during boosting with CoV. (a) Spleen cells were prepared from CoV-immunized and boosted mice at the indicated timepoints. Gated histograms show CoV Ab-positive spleen plasma cells (from gated plasma cells as shown in Fig. S1. The last panel (labeled in blue) shows OVA does not bind sPCs. (b) Boxplots show summarized CoV Ab-positive plasma cells. (c, d) Gated FACS plots show apoptotic portion in CoV-specific plasma cells. Violin plots show summarized apoptotic plasma cell counts. , compared with group wk3. Statistical methods: ANOVA followed by Dunnett’s test. Each group consists of 6 mice. Each dot in violin plots presents data obtained from one mouse.

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