Orcinol Glucoside Improves Senile Osteoporosis through Attenuating Oxidative Stress and Autophagy of Osteoclast via Activating Nrf2/Keap1 and mTOR Signaling Pathway

Figure 3

OG suppresses bone resorption in SAMP6 mice and osteoclast derived from RAW264.7 cells. (a) Representative Western blot analysis and the quantification of NFATc1, c-FOS, CTSK, and MMP9 expression in bone tissue of SAMP6 mice. (b) Osteoclasts were labeled with phalloidin (F-actin ring) and DAPI (nuclei). Scale bars: 20 μm. (c, d) The content of Ca²⁺ and CTX-1 in the coculture medium of osteoclast and bone slices. (e) Representative Western blot analysis and the quantification of NFATc1, c-FOS, CTSK, and MMP9 expression in osteoclast. Images presented are representative of ≥3 sections for each group or independent experiments, and each point represents . , vs. the indicated group.

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