Orcinol Glucoside Improves Senile Osteoporosis through Attenuating Oxidative Stress and Autophagy of Osteoclast via Activating Nrf2/Keap1 and mTOR Signaling Pathway

Figure 4

OG mitigates oxidative stress of bone tissue of SAMP6 mice and osteoclast derived from RAW264.7 cells. (a) Representative Western blot analysis and the quantification of NOX1, NOX2, NOX4, and SOD2 expression in bone tissue of mice. (b) Fluorometric quantification of ROS production was assayed using a multifunctional microplate reader; (c–i) the levels of NOX1, NOX2, NOX4, HO-1, GCS, NQO1, and G6PD were detected by ELISA kits. Images presented are representative of ≥3 sections for each group or independent experiments, and each point represents . , vs. the indicated group.

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