Curcumin Enhanced Ionizing Radiation-Induced Immunogenic Cell Death in Glioma Cells through Endoplasmic Reticulum Stress Signaling Pathways

Figure 6

Curcumin enhanced IR-mediated immune cell infiltration in tumor tissue. (a) Mice with normoxic or hypoxic tumor grafts were treated with curcumin and X-ray, and then, tumor and spleen cells were collected. CD3+ and CD8+ T cells were detected by flow cytometry. The number of CD8+ T cells per 10,000 cells was counted. (b) For immunohistochemical staining, immune subsets were shown using anti-CD4, CD8, CD11c, and FoxP3 antibodies in the spleen. (c) For immunohistochemical staining, immune subsets were shown using anti-CD4, CD8, CD11c, and FoxP3 antibodies in tumor tissues. (d) ER stress marker p-PERK, CHOP, p-IRE1α, and XBP1 expression were detected by immunohistochemical staining in tumor tissues. , , and vs. control; ^#, ^##, and ^### vs. IR.

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