G-Protein Coupled Receptor 35 Induces Intervertebral Disc Degeneration by Mediating the Influx of Calcium Ions and Upregulating Reactive Oxygen Species

Figure 1

Expression of GPR35 is correlated with IDD. (a) RT-PCR analysis suggests that NPCs have downregulation of Collagen II and upregulation of MMP-3 when compressed with 20 kPa for 4 h. (b) RNA-seq heat map suggests an upregulation of the GPR35 gene when NPCs are compressed at the above degenerative condition. (c) IHC staining suggests a gradual increase of GPR35 expression in human NPs along with higher degeneration levels. (The data are shown as the . for comparisons between two groups. One-way ANOVA and Tukey’s multiple comparison test were used for statistical analysis).

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