Rosiglitazone Ameliorates Spinal Cord Injury via Inhibiting Mitophagy and Inflammation of Neural Stem Cells

Figure 3

Overexpression of PINK1 counteracted relieved Rosi-induced suppression of mitophagy. (a, b) Confocal immunofluorescence images showing costaining of LC3B (red) and Tomm20 (green) (a) and mitoTimer (b) in NSCs, in which the forced PINK1 expression was induced by DOX or by the PBS as control and then subjected to Rosi. The forced PINK1 expression was controlled at the level similar to that without Rosi treatment. The statistical analyses were performed base on four randomly selected scopes under the confocal microscope. Scale bar represents 10 μm. (c, d) Flow cytometry results showing mitoTracker Green⁺/mitoTracker Red⁺ cells (c) and mitoTracker Green⁺/mitoSOX⁺ cells (d) with DMSO or Rosi treatments. The cells in the red rectangle are those of concern. Data represent three independent experiments.

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