Catalpol Enhances Random-Pattern Skin Flap Survival by Activating SIRT1-Mediated Enhancement of Autophagy

Figure 7

SIRT1 is involved in the protection of CAT against flap necrosis through the AMPK/mTOR signaling pathway. (a, b) Immunofluorescence staining and quantitative analysis of SIRT1-positive cells in the dermal layer in control and CAT groups after the surgery (scan bar, 10 μm). (c, d) Western blotting result and quantification of SIRT1 in each group at day 7 after surgery. (e, f) Western blotting and quantification analysis of SIRT1, AMPK, p-AMPK, mTOR, and p-mTOR in the flaps from in the control, CAT, and CAT+EX527 groups. (g, h) Western blotting and quantification analysis of autophagy-related proteins Beclin1, CTSD, LC3II, and P62 in the control, CAT, and CAT+EX527 groups. (i, j) Western blotting and quantification analysis of VEGFA, eNOS, Bax, and Bcl-2 in the control, CAT, and CAT+EX527 groups. Significance: and vs. the control group. ^# and ^## vs. the CAT group. Data were expressed as , .

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