Silibinin Alleviates Muscle Atrophy Caused by Oxidative Stress Induced by Cisplatin through ERK/FoxO and JNK/FoxO Pathways

Figure 4

SLI increased MyHC expression in the GA muscle, decreased MAFbx and Mstn expression, and regulated JNK and ERK to inhibit FOXO entry into the nucleus. (a) Using tubulin as an internal control, the expression of procaspase3 and active caspase3 was detected by Western blotting; the relative expression level of protein was detected by ImageJ software. Tubulin was used as internal controls and corrected to the NC group. Data are shown as , ; , versus group . (b) Using tubulin as an internal control, the expression of the MyHC, MAFbx, Mstn, JNK/FoxO, and ERK/FoxO signaling pathways was detected by Western blotting; the expression of nuclear FoxO3α using Lamin A as an internal control. (c) The relative expression level of protein was detected by ImageJ software. Tubulin and Lamin A were used as internal controls and corrected to the NC group. Data are shown as , ; , , versus group . (d) The qRT-PCR method to detect the expression of MyoG, Mstn, Fbxo32, and Ub mRNA in the GA muscle; RPS18 was used as an internal control. Data are shown as , ; , versus group .

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