Silibinin Alleviates Muscle Atrophy Caused by Oxidative Stress Induced by Cisplatin through ERK/FoxO and JNK/FoxO Pathways

Figure 6

Effect of SLI treatment on the ROS content of C2C12 myotube cells after DDP. (a) C2C12 myotube cells were stained with DCFH-DA and observed under a fluorescence microscope (). The green fluorescence indicated by the white arrow is ROS combined with DCFH-DA. (b) Relative fluorescence intensity of DCFH-DA staining. Data are shown as , ; , versus group DDP. (c) qRT-PCR method to detect the expression of Nrf2, SOD, CAT, and XDH mRNA in C2C12 myotube cells; RPS18 was used as an internal control. Data are shown as , ; , , versus group DDP. (d) Nrf2 expression was detected by Western blotting: total Nrf2 expression using tubulin as the internal control and nuclear Nrf2 expression using Lamin A as the internal control; the relative expression level of protein was detected using ImageJ software. Tubulin and Lamin A were used as internal controls and normalized to the NC group. Data are shown as , ; , , versus group DDP.

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