The Conflicting Role of Caffeine Supplementation on Hyperoxia-Induced Injury on the Cerebellar Granular Cell Neurogenesis of Newborn Rats

Figure 3

Quantification of (a) counts of colabeled calbindin and DAPI-positive Purkinje cells (PC) of the cerebellar molecular layer and (b) molecular layer (ML) length was performed for 3 days’ postnatal oxygen exposure (P3) and recovery (P3_P15) and 5 days’ postnatal oxygen exposure (P5) and recovery (P5_P15), respectively. Acute hyperoxia exposure (deep dark gray bars) decreased the number of Purkinje cells and depressed the dendrite length. Caffeine administration (gray bars) was a protective effect for calbindin-positive cells, but not for dendrite growth. Caffeine with normoxia (light gray bars) did not influence density and dendrite length of Purkinje cells, except at P5. Data are normalized to the level of rat pups exposed to normoxia at each time point (control 100%, white bars), and the 100% values are 1.4 (P3), 2.8 (P3_P15), 1.4 (P5), and 2.5 (P5_P15) length of the molecular layer or 14 (P3), 7.4 (P3_P15), 8.9 (P5), and 7.2 (P5_P15) cells per regions of lobules, respectively. -8/group. , , , and vs. control; ^#, ^##, and ^### vs. hyperoxia (ANOVA, Bonferroni’s post hoc test; Kruskal-Wallis, Dunn’s post hoc test).