The Conflicting Role of Caffeine Supplementation on Hyperoxia-Induced Injury on the Cerebellar Granular Cell Neurogenesis of Newborn Rats

Figure 5

Quantification of (a) Pax6 and (b) PCNA counts of colabelled positive cells of the cerebellar molecular layer was performed for 3 days’ postnatal oxygen exposure (P3) and recovery (P3_P15) and 5 days’ postnatal oxygen exposure (P5) and recovery (P5_P15), respectively. Acute hyperoxia exposure (P3 and P5; deep dark gray bars) decreased the number of granular cells (Pax6) and proliferating cells (PCNA). Caffeine (gray bars) was protective for Pax6-positive cells at P3 and P5 and for proliferating capacity at P3 with recovery until P15 (P3_P15). Caffeine with normoxia (light gray bars) did not influence density of granular cells and proliferation. Data are normalized to the level of rat pups exposed to normoxia at each time point (control 100%, white bars), and the 100% values are 38 (P3), 54.4 (P3_P15), 46.6 (P5), and 41.3 (P5_P15) Pax6+ cells per regions of lobules or 33 (P3), 37.1 (P3_P15), 65.7 (P5), and 22.3 (P5_P15) PCNA+ cells per region of lobules, respectively. -8/group. , , and vs. control; ^# and ^## vs. hyperoxia (ANOVA, Bonferroni’s post hoc test; Kruskal-Wallis, Dunn’s post hoc test; Brown-Forsythe, Dunnett’s post hoc test).