The Conflicting Role of Caffeine Supplementation on Hyperoxia-Induced Injury on the Cerebellar Granular Cell Neurogenesis of Newborn Rats

Figure 6

Quantification of cerebellar homogenates for granule cell precursor-associated mediators of (a) FGF8, (b) CycD2, (c) Sema6a, and (d) Lmx1α was performed with qPCR for 3 days’ postnatal oxygen exposure (P3) and recovery (P3_P15) and 5 days’ postnatal oxygen exposure (P5) and recovery (P5_P15), respectively. Acute hyperoxia exposure (deep dark gray bars) decreased the mRNA expression at P3 and/or P5. At P15, only the reduction of the proliferation marker CycD2 persisted. Caffeine failed to reverse the hyperoxia-induced effects (gray bars), except for CycD2 at P3_P15. Caffeine with normoxia (light gray bars) modulated the expression levels in a more differential manner. Data are normalized to the level of rat pups exposed to normoxia at each time point (control 100%, white bars). -8/group. , , , and vs. control; ^## and ^#### vs. hyperoxia (ANOVA, Bonferroni’s post hoc test; Kruskal-Wallis, Dunn’s post hoc test; Brown-Forsythe, Dunnett’s post hoc test).