The Conflicting Role of Caffeine Supplementation on Hyperoxia-Induced Injury on the Cerebellar Granular Cell Neurogenesis of Newborn Rats

Figure 8

Quantification of cerebellar homogenates for granule cell precursor- and granule cell-associated mediators of (a) Pax6, (b) Chd7, (c) NeuroD1, (d) NeuN, and (e) Prox1 was performed with qPCR for 3 days’ postnatal oxygen exposure (P3) and recovery (P3_P15) and 5 days’ postnatal oxygen exposure (P5) and recovery (P5_P15), respectively. Acute hyperoxia exposure (deep dark gray bars) decreased the mRNA expression at P3 and P5, except for Prox1. At P15, the reduction of Pax6, NeuroD1, and NeuN persisted after three-day oxygen exposure (P3_P15), as did the increased expression NeuroD1 and NeuN after five-day oxygen exposure (P5_P15). Caffeine rescued the transcription in comparison to hyperoxia-exposed group (gray bars) for Pax6, NeuN, and Prox1 at P3_15. Caffeine with normoxia (light gray bars) modulated the expression levels in a more differential manner. Data are normalized to the level of rat pups exposed to normoxia at each time point (control 100%, white bars). -8/group. , , , and vs. control; ^#, ^##, and ^### vs. hyperoxia (ANOVA, Bonferroni’s post hoc test; Kruskal-Wallis, Dunn’s post hoc test; Brown-Forsythe, Dunnett’s post hoc test).