tRNA-Derived Fragment tRF-5009A Regulates Autophagy and Degeneration of Cartilage in Osteoarthritis via Targeting mTOR

Figure 7

Effects of tRF-5009A binding to the 3-UTR of mTOR on translation. (a) qRT-PCR analysis of the expression of mTOR, COL2A1, and MMP13 in OA cells transfected with mTOR knockdown (KD) shRNAs or negative control (NC). (b) qRT-PCR, (c) western blotting, (d) mRFP-GFP-LC3 adenoviral double labelling, (f) transmission electron microscopy (TEM), (h) levels of reactive oxygen species (ROS), and (j) flow cytometry evaluation of the effects of cotransfection of cells with a mTOR KD-02 and a tRF-5009A inhibitor. (e) Quantification analysis of mRFP (red) and merge (yellow) dots per cell. (g) TEM images and (i) mean fluorescence intensity (MFI) of ROS. (k) Schematic illustration showing the flowchart of the prediction of the tRF-5009A-binding site in mTOR using a mutant. (l) Relative luciferase activity. All data were presented as the . ns: not significant, .

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