Shear-Induced ITGB4 Promotes Endothelial Cell Inflammation and Atherosclerosis

<div>ITGB4 mediates LSS-induced FAK, SRC, and NF<i>κ</i>B activation in HUVECs. (a) The protein levels of p-FAK, FAK, p-NF<i>κ</i>B, NF<i>κ</i>B, p-SRC, and SRC were measured by western blot in HUVECs treated with LSS for 0-24 h. (b) The protein levels of p-FAK, FAK, p-NF<i>κ</i>B, NF<i>κ</i>B, p-SRC, and SRC were measured by western blot isolated from static or low shear stress conditions transfected with scr siRNA or ITGB4 siRNA. (c) The representative images and location of NF<i>κ</i>B in HUVECs were assayed by immunofluorescence staining under static or low shear stress conditions transfected with scr siRNA or ITGB4 siRNA. Scale bars: 20 <i>μ</i>m. 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Oxidative Medicine and Cellular Longevity

fig4

Figure 4

Figure 4: Shear-Induced ITGB4 Promotes Endothelial Cell Inflammation and Atherosclerosis 