Functional Evaluation of KEL as an Oncogenic Gene in the Progression of Acute Erythroleukemia

Figure 2

KEL promotes cell proliferation and is responsible to drug resistance to JQ1. (a) Assessment of the proliferation of K562 cells and HEL cells transfected with KEL shRNA or lentivirus-mediated overexpression by CCK-8 assay. Data represents the (). (b) Representative display of dysregulated proteins detected by protein array. (c) Western blot validating the result of protein array. (d) IC50 value showing the drug sensitivity of K562 cells to 318 inhibitors. One resistant inhibitor above the red line and 14 sensitive ones below the blue line (https://www.cancerrxgene.org/). (e) Protein level change induced by JQ1 treatment and/or with KEL downregulation detected by western blot. (f) Knocking down KEL reversed the resistance of JQ1 in K562 as indicated by CCK-8 assays. Data represents the ().

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