Cholecystokinin Octapeptide Promotes ANP Secretion through Activation of NOX4–PGC-1α–PPARα/PPARγ Signaling in Isolated Beating Rat Atria

Figure 2

Effects of CCK-8s and PLC on cPLA2 expression and its role in ANP secretion and dynamics in isolated beating rat atria. (a) The protein expression levels of p-cPLA2 were detected by Western blot. (b, c) The statistics histograms of Western blot were expressed as band density normalized versus t-cPLA2. (d, f) Atrial ANP secretion by radioimmunoassay. (e, g) Atrial pulse pressure by RM6240BD. Cont: control; CCK-8s: sulfated CCK-8; Lo: Loxiglumid, an antagonist of CCK₁ receptor; YM: YM022, an antagonist of CCK₂ receptor; U: U-73122, an inhibitor of PLC; CAY: CAY10650, an inhibitor of cPLA2. Data were expressed as . (a–c) ; (d–g) . and vs. control period or group; ^## and ^### vs. CCK-8s period or group.

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