Cholecystokinin Octapeptide Promotes ANP Secretion through Activation of NOX4–PGC-1<i>α</i>–PPAR<i>α</i>/PPAR<i>γ</i> Signaling in Isolated Beating Rat Atria

<div>Effects of CCK-8s on PGC-1<i>α</i> expression and its role on ANP secretion and dynamics in isolated beating rat atria. (a) The protein expression levels of PGC-1<i>α</i> were detected by Western blot. (b, c) The statistics histograms of Western blot were expressed as band density normalized versus <i>β</i>-actin. (d) Atrial ANP secretion by radioimmunoassay. (e) Atrial pulse pressure by RM6240BD. Cont: control; CCK-8s: sulfated CCK-8; SB: SB239063, an antagonist of p38 MAPK; LY: LY294002, an inhibitor of Akt; SR: SR-18292, an antagonist of PGC-1<i>α</i>. 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27V0H384Z"></path></g></svg> vs. control period or group; <sup>##</sup><svg height="9.2729pt" id="M83" style="vertical-align:-0.6370001pt" version="1.1" viewbox="-0.0498162 -8.6359 44.8393 9.2729" width="44.8393pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M600 480C600 590 528 650 384 650H143L137 622C222 614 225 607 210 531L130 127C113 41 106 36 23 28L17 0H294L300 28C204 36 195 42 212 127L243 284L314 263C327 263 339 263 352 264C465 271 600 337 600 480ZM508 481C508 351 402 304 329 304C289 304 265 311 250 317L295 559C302 594 310 606 323 611C335 616 350 619 367 619C455 619 508 573 508 481Z"></path></g><g transform="matrix(.013,0,0,-0.013,11.658,0)"><path d="M512 -3V55L134 254V256L512 456V514L75 281V230L512 -3Z"></path></g><g transform="matrix(.013,0,0,-0.013,22.921,0)"><path d="M241 635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 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Oxidative Medicine and Cellular Longevity

fig5

Figure 5

Figure 5: Cholecystokinin Octapeptide Promotes ANP Secretion through Activation of NOX4–PGC-1<i>α</i>–PPAR<i>α</i>/PPAR<i>γ</i> Signaling in Isolated Beating Rat Atria 

Oxidative Medicine and Cellular Longevity

Cholecystokinin Octapeptide Promotes ANP Secretion through Activation of NOX4–PGC-1α–PPARα/PPARγ Signaling in Isolated Beating Rat Atria

Figure 5