Research Article
Nicotinamide Mononucleotide Ameliorates Cellular Senescence and Inflammation Caused by Sodium Iodate in RPE
Figure 1
(a–b) ARPE-19 cells were treated with different concentrations of NaIO3 for 24 h or 48 h. Cell viability was analyzed by CCK-8 assay (). (c–h) ARPE-19 cells were treated with 1 and 2.5 mM NaIO3 for 48 h. (c) The representative images of SA-β-gal staining in ARPE-19 cells; (). (d) Relative cell size distribution in ARPE-19 cells. X axis is FSC-A, which reflects cell size (). (e) p16 immunostaining in ARPE-19 cells; (). (f) Proteins were extracted from ARPE-19 cells. p21 protein levels were evaluated by western blot and β-tubulin was used as a loading control (). (g–h) Quantification of p16 and p21 mRNA expression in ARPE-19 cells (). GAPDH was used as a loading control. Data represent of at least three independent experiments, , , , compared versus control.
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