Nicotinamide Mononucleotide Ameliorates Cellular Senescence and Inflammation Caused by Sodium Iodate in RPE

Figure 2

(a) The representative images and the statistical results of immunofluorescent analysis of λ-H2AX foci in ARPE-19 treated with 1 and 2.5 mM NaIO₃ for 48 h; (). (b) The western blot and the statistical results of levels of λ-H2AX protein in NaIO₃ stimulated ARPE-19 cells (). (c) Cells were treated with NaIO₃ for 3 h. Cytosolic ROS levels were determined by flow cytometry (). (d) Cells were pretreated with 10 mM NAC for 24 prior to NaIO₃ treatment for 3 h. Cytosolic ROS levels were determined by flow cytometry (). (e) The representative images and the quantitative results of SA-β-gal staining in ARPE-19 cells treated as indicated; (). Data represent of at least three independent experiments, , , , , compared versus control or between the indicated groups.

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