Nicotinamide Mononucleotide Ameliorates Cellular Senescence and Inflammation Caused by Sodium Iodate in RPE

Figure 6

(a) The levels of Sirt1 and Acetyl-p65 proteins and the statistical results in senescent ARPE-19 cells. (b–c) Cell viability of ARPE-19 cells. The cells were pretreated with the indicated concentrations of Ex527 and SRT1720 for 24 h prior to treatment with 1 mM NaIO₃ for 48 h (). (d) The results of cell viability in cells co-cultured with NMN (1000 μM) and SRT1720 (10 μM) or with vehicle and SRT1720 (10 μM) for 24 h prior to treatment with 1 mM NaIO₃ for 48 h (). (e–g) The ARPE-19 cells were pretreated with NMN, NMN, and Ex527 or NMN and SRT1720 at the indicated concentrations for 24 h prior to treatment with 1 mM NaIO₃ for 48 h. (e) Cell viability was detected by CCK-8 assay (). (f) The levels of Sirt1 and Acetyl-p65 proteins in ARPE-19 cells with the indicated treatment were determined by western blot analysis (). (g) The representative images and statistical results of SA-β-gal staining assay; (). Data represent of at least three independent experiments, , , , , and the values represented the statistical significance of comparisons versus control except for additional labeling in the figures.

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