Nicotinamide Mononucleotide Ameliorates Cellular Senescence and Inflammation Caused by Sodium Iodate in RPE

Figure 7

(a–d) The ARPE-19 cells were pretreated with NMN, NMN, and Ex527 or NMN and SRT1720 at the indicated concentrations for 24 h prior to treatment with 1 mM NaIO₃ for 48 h. (a–b) Protein levels and statistical results of the indicated proteins in ARPE-19 cells. GAPDH or β-tubulin was used as loading control (). (c) Representative images of immunofluorescence of Tom20 in ARPE-19 cells; (). (d) Protein levels of mitochondrial dynamic proteins in ARPE-19 cells. GAPDH was used as loading control (). Data represent of at least three independent experiments, , , , , and the values represented the statistical significance of comparisons versus control except for additional labeling in the figures.

(a)

(b)

(c)

(d)