Dissecting Regulators of Aging and Age-Related Macular Degeneration in the Retinal Pigment Epithelium

Table 1

Proteins identified from spots changing with Aging (3 or less protein ID spots).

Spot no.†	value		Direction‡	Protein ID	Accession UniProt	Gene	Experimental MW/pI§	Theoretical MW/pI ¥	MSMS scaffold
									Sequence coverage	Unique peptides	Total spectra
A1	0.0319	0.09428	D	ATP synthase subunit d, mitochondrial	O75947	ATP5PD	21/5.6	18/5.30	30	4	6
				Ras-related protein Rab-18	Q9NP72	RAB18		23/5.11	17	3	3
A2	0.0148	0.1364	D	Transducin β-1	P62873	GNB1	33/5.5	37/6.00	45	9	17
				Retinaldehyde-binding protein 1	P12271	RLBP1		36/5.05	25	7	15
				Cathepsin D	P07339	CTSD		37.9/5.6 €	11	3	3
A3	0.0144	0.1286	D	HSP 60, mitochondrial	P10809	HSPD1	62/5.7	61/5.87	65	34	101
A4	0.0003	0.2497	D	NADH dehydrogenase iron sulfur protein 3, mitochondrial	O75489	NDUFS3	25.5/6.0	30/7.5	19	4	10
				Cathepsin D	P07339	CTSD		26.7/5.56 ₡	11	3	3
A5	0.0075	0.1933	D	Prohibitin	P35232	PHB	27/5.9	30/5.76	46	10	19
A6	0.0057	0.01576	D	Creatine kinase B	P12277	CKB	44/5.9	43/5.89	20	5	6
				Tripeptidyl-peptidase 1	O14773	TPP1		39.8/5.75 ∆	8	3	7
A9	0.0393	0.0931	I	Cathepsin D	P07339	CTSD	27.2/6.2	26.7/5.56 ₡	26	6	19
A12	0.0004	0.2391	D	Alpha enolase	P06733	ENO1	47.5/7.1	47.2/7.01	51	16	60
				HMG CoA synthase, mitochondrial	P54868	HMGCS2		52/8.16 δ	18	7	10
A14	0.0008	0.2104	D	HMG CoA synthase, mitochondrial	P54868	HMGCS2	47.5/7.2	52/8.16 δ	35	15	26
A15	0.0002	0.2558	D	Alpha enolase	P06733	ENO1	47/7.4	47/7.39	44	15	47
				HMG CoA synthase, mitochondrial	P54868	HMGCS2		52/8.16 δ	17	6	7
A16	0.0011	0.2945	D	Alpha enolase	P06733	ENO1	52/7.3	47/7.39	44	15	47
A17	0.0027	0.2233	D	Aldose reductase	P15121	AKR1B1	35/7.5	36/6.98	38	9	16
				Malate dehydrogenase, cytoplasmic	P40925	MDH1		36/7.39	21	5	5
				GAPDH	P04406	GAPDH		36/8.46	18	4	4
A18	0.0274	0.1082	I	Serotransferrin	P02787	TF	86/7.1	77/7.12	60	36	107
A19	0.0346	0.0955	D	Fructose-bisphosphate aldolase C	P09972	ALDOC	43/7.5	39/6.87	67	17	89
A20	0.0229	0.1228	D	Glutamine synthetase	P15104	GLUL	44.5/7.4	42/6.89	26	9	14
				Isocitrate dehydrogenase [NADP] cytoplasmic	O75874	IDH1		47/7.01	25	9	14
				Elongation factor Tu, mitochondrial	P49411	TUFM		50/7.61	13	4	4
A21	0.0314	0.3558	D	Phosphoglycerate mutase 1	P18669	PGAM1	29.6/7.1	29/7.18	19	5	11
A22	0.02	0.149	D	Transaldolase	P37837	TALDO1	42.5/7.1	38/6.81	19	6	6
A23	0.061	0.2286	I	Prohibitin	P35232	PHB	30.5/5.9	30/5.76	58	12	23
A24	0.0079	0.2124	I	Programmed cell death 6-interacting protein	Q8WUM4	PDCD6IP	97/6.9	96/6.52	13	9	10

† Spot number indicated on gel picture in Figure 2 left panel. Only spots with ≤3 proteins identifications are listed in this table. The remaining spots are listed in Table S2. ‡ Direction increasing (I) or decreasing (D) with age. § Experimental molecular weight (MW) of each spot was calculated based on the relative mobility of the MW markers and distance traveled on the 2D gel. Isoelectric point (pI) estimated from gel image. ¥ Theoretical MW and pI from Expasy MW/pI calculator (http://www.expasy.ch/tools). € Cathepsin D Active form (aa 65-412). ₡ Cathepsin D Heavy chain (aa169-412). ∆ TPP1 (aa 196-563). ⱡ 3,2-trans-enoyl-CoA isomerase (aa 42-302). trend. δ without mitochondrial signal sequence.