Resveratrol Treats UVB-Induced Photoaging by Anti-MMP Expression, through Anti-Inflammatory, Antioxidant, and Antiapoptotic Properties, and Treats Photoaging by Upregulating VEGF-B Expression

Figure 5

Effect of RES on antioxidant-related indicators in the skin of UVB-induced photoaging Hacat cells and ICR mice. (a) Changes in GSSH content in photoaging Hacat cells (). (b) Changes in SOD content in photoaging Hacat cells (). (c) Changes in ROS content in photoaging Hacat cells (, scale bar: 200 μM). (d) Relative expression content of GPX-4mRNA in photoaging Hacat cells using GADPH as an internal reference (). (e) Relative expression content of HO-1mRNA in photoaging Hacat cells using GADPH as an internal reference (). (f) Changes in nuclear Nrf2 protein levels in photoaging Hacat cells (). (g) Relative grey values of intranuclear Nrf2 protein in photoaging Hacat cells using Lamin B1 as an internal reference. (h) Relative mRNA expression of Nrf2 in the skin of photoaging ICR mice using GADPH as an internal reference (). (i) Relative mRNA expression of HO-1 in the skin of photoaging ICR mice using GADPH as an internal reference (). (j) Relative mRNA expression of NQO1 in the skin of photoaging ICR mice using GADPH as an internal reference (). (k) Relative mRNA expression of SOD1 in the skin of photoaging ICR mice using GADPH as an internal reference (). (l) Relative mRNA expression of GPX-4 in the skin of photoaging ICR mice using GADPH as an internal reference (). , , , compared to the control group; #, ##, ###, compared to the model group. C: control group; U: model group; R20, R40: RES concentration 20 and 40 μM drug-administered groups.

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