Hdac8 Inhibitor Alleviates Transverse Aortic Constriction-Induced Heart Failure in Mice by Downregulating Ace1

Figure 3

PCI34051 downregulates the expression of Hdac8 and inflammation-related genes in transverse aortic constriction (TAC) mice. (a–j) Cardiac and pulmonary mRNA levels of Hdac8, Il1b, Ccl2, Rela, and Nfkbia in the sham+vehicle, sham+PCI34051 (10 mg/kg bodyweight/day), TAC+vehicle, and TAC+PCI34051 (3, 10, or 30 mg/kg bodyweight/day) groups were determined using quantitative real-time polymerase chain reaction. (k) Cardiac expression levels of Hdac8, Ccl2, Rela, and Nfkbia; representative western blot images are shown. Actb was used as a loading control. (l–o) Quantification of Hdac8, Ccl2, Rela, and Nfkbia levels (–6 per group). (p) Pulmonary expression levels of Hdac8, Ccl2, Rela, and Nfkbia; representative western blot images. Actb was used as a loading control. (q–t) Quantification of Hdac8, Ccl2, Rela, and Nfkbia levels (–6 per group).

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