Hdac8 Inhibitor Alleviates Transverse Aortic Constriction-Induced Heart Failure in Mice by Downregulating Ace1

Figure 5

PCI34051 alleviates pulmonary congestion and fibrosis in transverse aortic constriction (TAC) mice. (a) The ratio of lung weight-to-bodyweight (LW/BW) in the sham+vehicle, sham+PCI34051 (10 mg/kg bodyweight/day), TAC+vehicle, and TAC+PCI34051 (3, 10, or 30 mg/kg bodyweight/day) groups (–6 per group) was determined. Representative images of mouse lungs (B). (b) Representative images of pulmonary tissues stained with hematoxylin and eosin.  μm. (c) Representative images of pulmonary tissues stained with Picrosirius red.  μm. (d) Pulmonary fibrosis was quantified using ImageJ software. (e–h) The mRNA levels of Col1a1, Fn1, Acta2, and Tgfb1 were evaluated using quantitative real-time polymerase chain reaction (–6 per group). (i–m) Representative western blots and quantification of Fn1, Acta2, Tgfb1, p-Smad2/3, and Smad2/3 levels in the pulmonary tissues (–6 per group).

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