Propofol via Antioxidant Property Attenuated Hypoxia-Mediated Mitochondrial Dynamic Imbalance and Malfunction in Primary Rat Hippocampal Neurons

Figure 5

Propofol alleviated hypoxia-mediated expression and phosphorylation of Drp1. The upper panel was a representative experiment, and the lower panel was the summary of densitometric data from 5 separate experiments. GAPDH served as loading control. (a) Hypoxia induced the protein expression of Drp1 (left) but not Fis1 (right), and hypoxia-mediated Drp1 expression was attenuated by 50 μM propofol, 0.1 mM α-tocopherol, and 40 μM ebselen. Data were expressed as normalized ratio of protein band density of Drp1 or Fis1 against GAPDH and were presented as . (b) Hypoxia induced the phosphorylation of Drp1 at ser 616 (p-Drp1^ser616), while reduced the phosphorylated of Drp1 at ser 637 (p-Drp1^ser637), which were both attenuated by propofol, α-tocopherol, and ebselen. Data were expressed as normalized ratio of protein band density of phosphorylated Drp1 against Drp1, which was normalized with GAPDH, and were presented as . (c) Hypoxia, propofol, α-tocopherol, and ebselen had no effect on the expression of Opa1 (left), Mfn1 (middle) or Mfn2 (right). Data were expressed as normalized ratio of protein band density of Opa1, Mfn1, or Mfn2 against GAPDH, and were presented as .

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