Propofol via Antioxidant Property Attenuated Hypoxia-Mediated Mitochondrial Dynamic Imbalance and Malfunction in Primary Rat Hippocampal Neurons

Figure 6

Hypoxia- and propofol-modulated Drp1 expression and mitochondrial dynamics were mediated through HIF-1α. The upper panel was a representative experiment, and the lower panel was the summary of densitometric data from 5 separate experiments. GAPDH served as loading control. Data were expressed as normalized ratio of protein band density of target protein against GAPDH and were presented as . (a) Hypoxia-induced translocation of HIF-1α from cytoplasm to nucleus was abolished by propofol, α-tocopherol, and ebselen. (b) Hypoxia-induced Drp1 expression was mitigated by HIF-1α inhibitors. (c) Hypoxia-mediated mitochondrial fragmentation was attenuated by HIF-1α inhibitors. Data were presented as the percentage of of treated neurons compared with that of untreated control neurons, which was set as 100%, and represents the number of independent repeats.

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