Controlled Hemorrhage Sensitizes Angiotensin II-Elicited Hypertension through Activation of the Brain Renin-Angiotensin System Independently of Endoplasmic Reticulum Stress

Table 1

Primer sequences for real-time PCR.

Gene	Forward primer	Reverse primer	Product size (bp)
GAPDH	TGACTCTACCCACGGCAAGTTCAA	ACGACATACTCAGCACCAGCATCA	141
Renin	CTGCCACCTTGTTGTGTGAG	ACCTGGCTACAGTTCACAACG	154
ACE1	GTGTTGTGGAACGAATACGC	CCTTCTTTATGATCCGCTTGA	187
AT1-R	CTCAAGCCTGTCTACGAAAATGAG	GTGAATGGTCCTTTGGTCGT	188
ACE2	TTAAGCCACCTTACGAGCCTC	GCCAATGTCCATGGAGTCAT	170
Mas-R	TGTGGGTGGCTTTCGATT	CCCGTCACATATGGAAGCAT	159
TNF-α	GCCGATTTGCCACTTCATAC	AAGTAGACCTGCCCGGACTC	209
IL-6	GCCTATTGAAAATCTGCTCTGG	GGAAGTTGGGGTAGGAAGGA	160
IL-1β	AGCAACGACAAAATCCCT GT	GAAGACAAACCGCTTTTCCA	209
GRP 78	TTCCGAGGAACACTGTGGTG	GTCAGGGGTCGTTCACCTTC	109

ACE: angiotensin-converting enzyme; AT1-R: angiotensin II type 1 receptor; Mas-R: angiotensin-(1–7) receptor; TNF-α: tumor necrosis factor-α; IL-1β: interleukin-1β; IL-6: interleukin-6; GRP 78: the glucose-regulated protein 78.