A20 Is Increased in Fetal Lung in a Sheep LPS Model of Chorioamnionitis

Figure 3

Repeated LPS exposure inhibits single LPS-induced proinflammatory cytokine and A20 mRNA expressions in HPMEC-ST1.6R cells. HPMEC-ST1.6R cells were incubated with LPS (100 ng/mL) for 4 h (A, black column) or cells were first exposed to LPS (100 ng/mL) for 4 h, then LPS was washed off and after a 24 h recovery period the cells were exposed to LPS (100 ng/mL) for 4 h a second time (B, white column). mRNA levels of IL-1β, IL-6, IL-8, TNF-α, and A20 were measured by real-time PCR and normalized to β-actin mRNA level. of independent experiments are shown. compared to untreated cells, ^# compared to respective 4 h single treated cells.