A Linarin Derivative Protects against Ischemia-Induced Neuronal Injury in Mice by Promoting Cerebral Blood Flow Recovery via KDELR-Dependent CSPG4 Activation

Figure 2

Delivery of BLR improved short-term stroke outcomes. The mice were subjected to 30min MCAO and 24h reperfusion and treated with BLR at the dose of 4, 20, and 40mg/kg or vehicle via intraperitoneal at 1h after reperfusion onset; the series of battery behavior tests were performed 24h post stroke. (a) Schematic diagram of the experimental design. (b) Body weight was monitored over time. ^## compared with the sham group ( per group). (c) LDF recorded the CBF changes in ischemic core of the MCA territory ( per group). ^## compared with the sham group. (d) Representative TTC-stained images of brain slices. (e) Infarct volume of TTC-stained brain slices was quantitated ( per group). ^### compared with the sham group. versus MCAO group. (f) Neurological defects were evaluated by neurologic scores ( per group). ^### compared with the sham group. versus MCAO group. (g) Open field and (h) rotarod tests were evaluated presurgery (day 0) at baselines and at 24 hours after MCAO ( per group). Data were analyzed by two-way ANOVA, followed by two-tailed unpaired -tests. ^# compared with the sham group. versus MCAO group.

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