A Linarin Derivative Protects against Ischemia-Induced Neuronal Injury in Mice by Promoting Cerebral Blood Flow Recovery via KDELR-Dependent CSPG4 Activation

Figure 4

BLR inhibited cerebral blood flow decline in ischemic zone induced by MCAO. (a) Cerebral blood flow was detected by LSCI in entire MCAO process (including pre-MCAO, during MCAO, and post-MCAO) ( per group). Representative laser speckle contrast images of surface cortical CBF. Red oval represents ROI of ipsilateral hemisphere; green oval represents ROI area of contralateral. (b) Blood flow index changes (lpsi ROI/control ROI) of CBF in MCAO or BLR treatment ( per group). compared with the MCAO group. (c) 3D volume rendering of the vascular architecture in ischemic stroke. Brain transparent was realized by cubic clarity in the mouse MCAO model. The brain of the mice was clarified by CUBIC ( per group) after MCAO and BLR treatment. The ischemic regions labeling with red circle were detected by ultramicroscope ( per group). White arrows point to the areas which represent the blood vessels. Scale  μm. (d) Representative immunofluorescence images of CD31 ( per group) to evaluate the capillary density in ischemic brain tissue. Scale μm.

(a)

(b)

(c)

(d)