Aβ1–40 Oligomers Trigger Neutrophil Extracellular Trap Formation through TLR4- and NADPH Oxidase-Dependent Pathways in Age-Related Macular Degeneration

Figure 4

Extracellular Aβ1–40 oligomers induce NETs formation. (a) Western blot analysis of H3-cit expression in neutrophils following the treatment described above. (b) Neutrophils were incubated with PMA (200 nM), Aβ1–40 oligomers (10 μM), Aβ40–1 (10 μM), or the control for 4 h at 37°C. DAPI was used to label extracellular DNA. H3-cit was immunolabelled with an antibody. . (c and d) Measurements of extracellular DNA and NE levels indicated the presence of NETs. (e) ROS production induced by treatment at different concentrations, as quantified by preloading with the DCFH-DA probe and measured with a microplate reader. , .

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