Research Article

[Retracted] Mild Hypothermia Promotes Ischemic Tolerance and Survival of Neural Stem Cell Grafts by Enhancing Global SUMOylation

Figure 1

Effect of 12 h hypoxia on NSCs. (a) LDH contents of control and hypoxia groups were measured by ELISA. (b) Apoptosis was measured by flow cytometry of the two groups. Data shown indicate (). (c) Expression of NSE or nestin (red fluorescent signal) and GFAP (green fluorescent signal) in NSCs of the control and hypoxia groups was examined by immunofluorescence; DAPI (blue) was used to stain cell nuclei (scale bar, 20 μm). (d) Oct4 and SOX2 protein expression was examined by western blotting. GAPDH was used for normalization. Data shown indicate (). (e) ECAR analysis of the glycolytic capacity of the control and hypoxic NSCs. Data shown indicate (); compared with the control group.
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