[Retracted] Mild Hypothermia Promotes Ischemic Tolerance and Survival of Neural Stem Cell Grafts by Enhancing Global SUMOylation

Figure 5

UBC9 interference reduced NSC stemness and tolerance to hypoxia. (a) and (b) Expression of SUMO1 and SUMO2/3 conjugates, free SUMO1 and SUMO2/3, UBC9, Oct4, and SOX2 in NSCs after UBC9 interference. (c) Quantitative data were normalized to GAPDH and are shown as (). (d) Expression of NSE or nestin (red fluorescent signal) and GFAP (green fluorescent signal) in NSCs of the siUBC9 or Con groups after 12 h hypoxia was examined by immunofluorescence; DAPI (blue) was used to stain cell nuclei (scale bar, 10 μm). (e) LDH contents were measured by ELISA, and data shown indicate (). (f) Western blotting to detect the expression of cleaved caspase-3 in NSCs. Quantitative analysis: cleaved caspase-3 expression was normalized to GAPDH and is expressed as (). and vs. control.

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