miR-29a-3p in Exosomes from Heme Oxygenase-1 Modified Bone Marrow Mesenchymal Stem Cells Alleviates Steatotic Liver Ischemia-Reperfusion Injury in Rats by Suppressing Ferroptosis via Iron Responsive Element Binding Protein 2

Figure 1

Inhibition of ferroptosis can reduce steatotic liver IRI. Serum ALT (a) and AST (b) levels in IRI, with or without Fer-1 treatment. (c) Pathological HE staining of steatotic liver IRI; Fer-1 significantly reduced liver pathological injury. (d) Suzuki score of steatotic liver IRI. (e) TUNEL staining of steatotic liver IRI; Fer-1 significantly decreased the number of TUNEL positive cells (f). The contents of Fe²⁺ (g), MDA (h), and GSH (i) in IRI, with or without Fer-1, were detected using colorimetry. (j) The Ptgs2 mRNA level. (k) GPX4 protein level determined using western blotting. (l) Representative images of 4-HNE immunohistochemical staining. (m) Semiquantitative analysis of (k). (n) Liver transmission electron microscopy showing that the number of mitochondrial cristae decreased, the membrane density increased, and membrane was ruptured in steatotic liver IRI and was improved by Fer-1. per group. Data are presented as the . ; .