miR-29a-3p in Exosomes from Heme Oxygenase-1 Modified Bone Marrow Mesenchymal Stem Cells Alleviates Steatotic Liver Ischemia-Reperfusion Injury in Rats by Suppressing Ferroptosis via Iron Responsive Element Binding Protein 2

Figure 3

HO-1/BMMSCs could suppress ferroptosis of steatotic liver IRI and this modulation could be mediated by IREB2. Evaluation of ferroptosis in steatotic liver IRI with BMMSCs or HO-1/BMMSC treatment. (a) MDA content detected using colorimetry. (b) GSH content detected using colorimetry. (c) The Ptgs2 mRNA level. (d) Representative TEM images showing that HO-1/BMMSCs improved the ferroptosis morphology of mitochondria (mitochondrial cristae decrease, membrane density increased, and membrane rupture). (e) Representative immunohistochemical images of 4-HNE. (f) GPX4 protein levels assessed using western blotting. (g) Semiquantitative analysis of (f). (h) Representative immunohistochemical images of IREB2 showing that that positive expression was increased significantly in IRI and decreased by HO-1/BMMSC treatment. (i) IREB2/FTH1/TFR1 protein levels assessed using western blotting. (j) Semiquantitative analysis of (i). (k) The Fe²⁺ level detected using colorimetry. per group. Data are presented as the . ; .

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