miR-29a-3p in Exosomes from Heme Oxygenase-1 Modified Bone Marrow Mesenchymal Stem Cells Alleviates Steatotic Liver Ischemia-Reperfusion Injury in Rats by Suppressing Ferroptosis via Iron Responsive Element Binding Protein 2

Figure 5

HO-1/BMMSC-mediated inhibition of ferroptosis and alleviation of SHP-HR injury could be mediated by regulating the protein levels of IREB2. Evaluation of ferroptosis and cell injury in SHP-HR with BMMSCs or HO-1/BMMSCs. (a) Representative immunofluorescence images of IREB2 showing that positive expression increased significantly in HR and was decreased by HO-1/BMMSC treatment. (b) IREB2/FTH1/TFR1/GPX4 protein levels assessed using western blotting. (c) Ireb2 mRNA level detected using qRT-PCR. (d) Fe²⁺ level detected using colorimetry. (e) The lipid ROS level detected using C11-BODIPY and flow cytometry. (f) Quantitative analysis of C11-BODIPY fluorescence intensity. (g) Cell viability detected using a CCK-8 kit. (h) Cell mitochondrial membrane potential detected using JC-1 staining and flow cytometry. (i) Quantitative analysis of (h). (j) Cell death ratio detected using PI staining and flow cytometry. (k) Quantitative analysis of (j). per group. Data are presented as the . ; .

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