miR-29a-3p in Exosomes from Heme Oxygenase-1 Modified Bone Marrow Mesenchymal Stem Cells Alleviates Steatotic Liver Ischemia-Reperfusion Injury in Rats by Suppressing Ferroptosis via Iron Responsive Element Binding Protein 2

Figure 8

Loss of miR-29a-3p in HBM-exos results in loss of regulation of IREB2-mediated ferroptosis and the protective function of SHP-HR. Evaluation of ferroptosis and cell injury in , with or without the miR-29a-3p inhibitor treatment. (a) The miR-29a-3p inhibitor significantly reduced the elevated level of miR-29a-3p in the coculture system of HBM-exos and SHP-HR. (b) IREB2/FTH1/TFR1/GPX4 protein levels assessed using western blotting, showing that miR-29a-3p inhibitor eliminated the regulation by HBM-exos on the protein levels of IREB2/FTH1/TFR1/GPX4. (c) The Fe²⁺ level detected using colorimetry. (d) Representative images of IREB2 and FTH1 immunofluorescence costaining. (e) Representative images of IREB2 and TFR1 immunofluorescence costaining. (f) Lipid ROS levels detected using C11-BODIPY and flow cytometry. (g) Cell mitochondrial membrane potential detected using JC-1 staining and flow cytometry. (h) Quantitative analysis of (g). (i) Cell death ratio detected using PI staining and flow cytometry. (j) Quantitative analysis of (i). per group. Data are presented as the . ; .

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