The Role of CD147 in Pathological Cardiac Hypertrophy Is Regulated by Glycosylation

Figure 4

Defective CD147 glycosylation improved profibrotic effects of CD147 in mouse hearts after transverse aortic constriction (TAC). (a) Representative images of heart sections from the different groups stained with Masson’s trichrome. (b) Representative images of heart sections stained with PSR. (c) Quantification of the percentage of the left ventricular fibrotic area ( mice/group with 4–6 visual fields per mouse). (d) Representative western blot analysis of collagen-3 (COL-3) and α-SMA in cardiac tissue from the indicated groups (). (e) Real-time polymerase chain reaction analysis of collagen-1, collagen-3, Ctgf, and Mmp2 mRNA expression levels in the indicated groups (). vs. the sham group; vs. the sham group; vs. the sham group; vs. the sham group; ^# vs. the vehicle group; ^## vs. the vehicle group; ^### vs. the vehicle group; ^#### vs. the vehicle group; ^✟ vs. the OE group; ^✟✟ vs. the OE group, ^✟✟✟ vs. the OE group. PSR: picrosirius red; COL-3: collagen-3; α-SMA: α-smooth muscle actin; CTGF: connective tissue growth factor; MMP2: matrix metalloproteinase 2.

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