Patchouli Alcohol Inhibits D-Gal Induced Oxidative Stress and Ameliorates the Quality of Aging Cartilage via Activating the Nrf2/HO-1 Pathway in Mice

Table 1

List of primer sequences for RT-qPCR.

Gene	Forward primer	Reverse primer
Col2a1	5-GGAATTTGGTGTGGACATAGGG-3	5-GGTCAGGTCAGCCATTCAGT-3
Mmp13	5-AGTTGACAGGCTCCGAGAAA-3	5-GGCACTCCACATCTTGGTTT-3
Nrf2	5-GTAGATGACCATGAGTCGCTTGCC-3	5-CTTGCTCCATGTCCTGCTCTATGC-3
Hmox1	5-ACCGCCTTCCTGCTCAACATTG-3	5-CTCTGACGAAGTGACGCCATCTG-3
Sod1	5-CCACTGCAGGACCTCATTTT-3	5-CACCTTTGCCCAAGTCATCT-3
Cat	5-AATATCGTGGGTGACCTCAA-3	5-CAGATGAAGCAGTGGAAGGA-3
Gss	5-CAGCCAGAACCCAGCCTTCC-3	5-GCGATTCAGGCCCAGGAACA-3
Keap1	5-CAACTTCGCTGAGCAGATTGGC-3	5-TGATGAGGGTCACCAGTTGGCA-3
Nqo1	5-CTCGTAGCAGGATTTGCC-3	5-GAAGCCACAGAAACGCA-3
Tp53	5-GCATGAACCGCCGACCTATCC-3	5-CCCAGGGCAGGCACAAACAC-3
Cdkn1a/p21^Cip1/Waf1	5-TCCTGGTGATGTCCGACCTGTTC-3	5-CGGCGCAACTGCTCACTGTC-3
Cdkn2a/p16^INK4a	5-GTCGCAGGTTCTTGGTCAC-3	5-TCTGCACCGTAGTTGAGCAG -3
Acan	5-TAGAGGATGTGAGTGGTCTT-3	5-TCCACTAAGGTACTGTCCAC-3
Gapdh	5-GAGTCAACGGATTTGGTCGT−3	5-TTGATTTTGGAGGGATCTCG−3

Col2a1, collagen 2a1; Mmp13, matrix metalloproteinase 13; Nrf2, nuclear factor-erythroid 2-related factor 2; Hmox1, heme oxygenase-1; Sod1, superoxide dismutase 1; Cat, catalase; Gss, glutathione synthetase; Keap1, Kelch-like ECH-associated protein 1; Nqo1, NAD(P)H quinone dehydrogenase 1; Tp53, tumor protein p53; Cdkn1a/p21^Cip1/Waf1, Cyclin-Dependent Kinase Inhibitor 1A; Cdkn2a/p16^INK4a, Cyclin-Dependent Kinase Inhibitor 2A; Acan, aggrecan; Gapdh, glyceraldehyde phosphate dehydrogenase gene.