Small Noncoding RNAs Contribute to Sperm Oxidative Stress-Induced Programming of Behavioral and Metabolic Phenotypes in Offspring

<div>Composition of sncRNAs in sperm with or without oxidative stress. (a) Subcellular fractionation of miRNAs, tsRNAs, rsRNAs, and piRNAs in Con and OS sperm. (b) Heat map of differentially expressed 29-34 nt 5<span class="nowrap"><svg height="11.4859pt" id="M16" style="vertical-align:-0.04979992pt" version="1.1" viewbox="-0.0498162 -11.4361 3.89517 11.4859" width="3.89517pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M310 541L304 571C290 586 211 619 185 610L80 76L131 52L310 541Z"></path></g></svg>-</span>tsRNAs in sperm. (c) Northern blot analysis of 5<span class="nowrap"><svg height="11.4859pt" id="M17" style="vertical-align:-0.04979992pt" version="1.1" viewbox="-0.0498162 -11.4361 3.89517 11.4859" width="3.89517pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M310 541L304 571C290 586 211 619 185 610L80 76L131 52L310 541Z"></path></g></svg>-</span>tsRNA-Glu (shown by arrowheads) in sperm.</div>

Oxidative Medicine and Cellular Longevity

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Figure 1

Figure 1: Small Noncoding RNAs Contribute to Sperm Oxidative Stress-Induced Programming of Behavioral and Metabolic Phenotypes in Offspring 